Journal: PLOS One
Article Title: Microfluidic isolation and release of live disseminated breast tumor cells in bone marrow
doi: 10.1371/journal.pone.0319392
Figure Lengend Snippet: (A) Genes were all highly expressed in two cancer cell lines and barely expressed in BM samples. Specifically, there were no detectable levels of EGFR and EpCAM in both BM samples. MDA: MDA-MB-231; MCF7: MCF-7; BM1, BM2: bone marrow cells from healthy donors #1 and #2; BM1+1, +5, +50: 1, 5, 50 MDA-MB-231 cells were spiked into 1 million BM cells from healthy donor #1. (B) EGFR was highly expressed in MDA-MB-231 cells, an observation that aligned with the literature. Nucleated human BM cells were used as control. Streptavidin-PE was used to label biotinylated antibodies.
Article Snippet: Biotinylated antibody against epithelial cell adhesion molecule (anti-EpCAM) (eBioscience, Carlsbad, CA, USA) and biotinylated antibody against epidermal growth factor receptor (anti-EGFR, Research Grade Cetuximab Biosimilar) (R&D Systems, Minneapolis, MN, USA) were used as the tumor cell capture agents immobilized on the surface of the microchannels.
Techniques: Control